Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.
Human Semaphorin 4C Antibody (660016)
R&D Systems | Catalog # MAB6125
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Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Ala21-Gly663 (predicted)
Accession # Q9C0C4
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Semaphorin 4C Antibody (660016)
Detection of Human Semaphorin 4C by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Semaphorin 4C Antibody (660016)
Western Blot
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Semaphorin 4C
Alternate Names
Gene Symbol
UniProt
Additional Semaphorin 4C Products
Product Documents for Human Semaphorin 4C Antibody (660016)
Certificate of Analysis
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Product Specific Notices for Human Semaphorin 4C Antibody (660016)
For research use only
Related Research Areas
Citations for Human Semaphorin 4C Antibody (660016)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
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