Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).
Human Serpin A1/alpha 1-Antitrypsin Antibody (1019625)
R&D Systems | Catalog # MAB12681
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1019625
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Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human Serpin A1/alpha 1-Antitrypsin
Glu25-Lys418
Accession # P01009
Glu25-Lys418
Accession # P01009
Specificity
Detects human SerpinA1/ alpha 1-Antitrypsin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Serpin A1/alpha 1-Antitrypsin Antibody (1019625)
Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot.
Western blot shows lysates of human lung tissue, human kidney tissue, HT-29 human colon adenocarcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB12681) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin A1/a1‑Antitrypsin by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/a1‑Antitrypsin at approximately 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin A1/a1‑Antitrypsin Monoclonal Antibody (Catalog # MAB12681). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Serpin A1/alpha 1-Antitrypsin Antibody (1019625)
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
2 µg/mL
Sample: Human lung tissue, Human kidney tissue, HT‑29 human colon adenocarcinoma cell line, and Huh‑7 human hepatoma cell line
Sample: Human lung tissue, Human kidney tissue, HT‑29 human colon adenocarcinoma cell line, and Huh‑7 human hepatoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin A1/alpha 1-Antitrypsin
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Barbour, K.W. et al. (2002) Genomics 80:515.
- Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.
Alternate Names
A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor
Entrez Gene IDs
5265 (Human)
Gene Symbol
SERPINA1
UniProt
Additional Serpin A1/alpha 1-Antitrypsin Products
Product Documents for Human Serpin A1/alpha 1-Antitrypsin Antibody (1019625)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin A1/alpha 1-Antitrypsin Antibody (1019625)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
