Human sFRP-2 Antibody (755644)

R&D Systems | Catalog # MAB6838

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 755644
View all sFRP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-2
Leu25-Cys295
Accession # Q96HF1

Specificity

Detects human sFRP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) sFRP-2 is observed and no cross-reactivity with recombinant human (rh) sFRP-1 or rhsFRP-5 is observed. In Western blots, no cross-reactivity with rhsFRP-1, -4, -5, or rmsFRP-2 is observed. 100% cross-reactivity with rhsFRP-3 is observed under non-reducing conditions only.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human sFRP-2 Antibody (755644)

Detection of Human sFRP-2 antibody by Western Blot.

Detection of Human sFRP‑2 by Western Blot.

Western blot shows lysates of human adipose tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human sFRP-2 Monoclonal Antibody (Catalog # MAB6838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for sFRP-2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human sFRP-2 Antibody (755644)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human adipose tissue and human lung tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-2

Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1, 2). Mature human sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with mouse and rat sFRP-2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP-induced effects (8, 10). sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP-2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  4. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  5. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.

Long Name

Secreted Frizzled Related Protein 2

Alternate Names

SARP-1, SDF-5, sFRP2

Entrez Gene IDs

6423 (Human); 20319 (Mouse)

Gene Symbol

SFRP2

UniProt

Q96HF1

Additional sFRP-2 Products

Product Documents for Human sFRP-2 Antibody (755644)

Certificate of Analysis

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Product Specific Notices for Human sFRP-2 Antibody (755644)

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

Citations for Human sFRP-2 Antibody (755644)

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