Human SOX9 Antibody [Biotin]
R&D Systems | Catalog # BAF3075
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Met1-Lys151
Accession # P48436
Specificity
In Western blots, approximately 5% cross-reactivity with recombinant human (rh) SOX10 is observed and less than 2% cross-reactivity with rhSOX2, rhSOX3, rhSOX7, rhSOX14, rhSOX17, and rhSOX21 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SOX9 Antibody [Biotin]
SOX9 in BGO1V Human Embryonic Stem Cells.
SOX9 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human SOX9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human SOX9 Antibody [Biotin]
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated to osteocytes, adipocytes, and chondrocytes using Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006) and immersion fixed BG01V human embryonic stem cells differentiated to early proximal lung progenitor
Sample: Immersion fixed human mesenchymal stem cells differentiated to osteocytes, adipocytes, and chondrocytes using Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006) and immersion fixed BG01V human embryonic stem cells differentiated to early proximal lung progenitor
Western Blot
0.1 µg/mL
Sample: Recombinant Human SOX9
Sample: Recombinant Human SOX9
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SOX9
Long Name
Transcription Factor SOX9
Alternate Names
campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9
Entrez Gene IDs
6662 (Human)
Gene Symbol
SOX9
UniProt
Additional SOX9 Products
Product Documents for Human SOX9 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SOX9 Antibody [Biotin]
For research use only
Related Research Areas
Citations for Human SOX9 Antibody [Biotin]
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
