Human STIM1 Antibody (705129)

R&D Systems | Catalog # MAB69761

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 705129
View all STIM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STIM1
Leu23-Thr182
Accession # Q13586

Specificity

Detects human STIM1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human STIM2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human STIM1 Antibody (705129)

STIM1 antibody in Human Brain by Immunohistochemistry (IHC-P).

STIM1 in Human Brain.

STIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human STIM1 Monoclonal Antibody (Catalog # MAB69761) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human STIM1 Antibody (705129)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Reviewed Applications

Read 1 review rated 5 using MAB69761 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STIM1

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel Orai1, increasing Ca2+ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Entrez Gene IDs

6786 (Human); 20866 (Mouse); 361618 (Rat)

Gene Symbol

STIM1

UniProt

Q13586

Additional STIM1 Products

Product Documents for Human STIM1 Antibody (705129)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STIM1 Antibody (705129)

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for Human STIM1 Antibody (705129) (1)

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  • Human STIM1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung cancer tissue
    Species: Human
    Verified Customer | Posted 03/03/2022
    Human STIM1 Antibody (705129) MAB69761

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