Human SYK (SH2D2 Domain) Antibody (720402)

R&D Systems | Catalog # MAB7166

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 720402
View all SYK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SYK (SH2D2 Domain)
Trp168-Cys259
Accession # P43405

Specificity

Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SYK (SH2D2 Domain) Antibody (720402)

Detection of Human and Mouse SYK (SH2D2 Domain) antibody by Western Blot.

Detection of Human and Mouse SYK (SH2D2 Domain) by Western Blot.

Western blot shows lysates of EL-4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for SYK (SH2D2 Domain) at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Raji human Burkitt's lymphoma cell line 0.2 antibody by Simple WesternTM.

Detection of Raji human Burkitt's lymphoma cell line 0.2 by Simple WesternTM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse SYK antibody by Simple WesternTM.

Detection of Mouse SYK by Simple WesternTM.

Simple Western lane view shows lysates of EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human SYK Specificity Using Knockout Cell Line.

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and SYK knockout THP-1 cell line (KO). Nitrocellulose membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for SYK at approximately 68 kDa (as indicated) in the parental THP-1 cell line, but is not detectable in knockout THP-1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for Human SYK (SH2D2 Domain) Antibody (720402)

Application
Recommended Usage

Knockout Validated

SYK is specifically detected in the parental THP-1 cell line, but is not detectable in knockout THP-1 cell line. 

Simple Western

20 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and EL‑4 mouse lymphoblast cell line

Western Blot

2 µg/mL
Sample: EL‑4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SYK

SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.

Long Name

Spleen Tyrosine Kinase

Alternate Names

DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK

Entrez Gene IDs

6850 (Human); 20963 (Mouse); 25155 (Rat)

Gene Symbol

SYK

UniProt

P43405

Additional SYK Products

Product Documents for Human SYK (SH2D2 Domain) Antibody (720402)

Certificate of Analysis

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Product Specific Notices for Human SYK (SH2D2 Domain) Antibody (720402)

For research use only

Citations for Human SYK (SH2D2 Domain) Antibody (720402)

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