Human TBX2 Antibody (393725)

R&D Systems | Catalog # MAB5040

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 393725
View all TBX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207

Specificity

Detects endogenous human TBX2 in Western blots at approximately 75 kDa.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human TBX2 Antibody (393725)

Detection of Human TBX2 antibody by Western Blot.

Detection of Human TBX2 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. PVDF membrane was probed with 0.1 µg/mL of Human TBX2 Monoclonal Antibody (Catalog # MAB5040) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TBX2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TBX2 Antibody (393725)

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: BG0IV human embryonic stem cells, HEK293 human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX2

TBX2 (T-box transcription factor 2) is a member of a family of transcription factors that share the T-box DNA binding domain. TBX2, as well as other TBX proteins, is involved in remodeling of the heart myocardium through a complex spatial and temporal regulation. TBX2 is associated with tumorigenesis and anti-senescence properties by affecting several cyclin-dependent kinase inhibitors. The full-length protein contains 702 amino acids with a DNA binding domain from aa 99 to aa 277.

Long Name

T-box Transcription Factor 2

Alternate Names

FLJ10169, T-box 2, T-box protein 2, T-box transcription factor TBX2

Entrez Gene IDs

6909 (Human); 21385 (Mouse); 303398 (Rat)

Gene Symbol

TBX2

UniProt

Q13207

Additional TBX2 Products

Product Documents for Human TBX2 Antibody (393725)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX2 Antibody (393725)

For research use only

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