Human TDAG8/GPR65 Antibody (976128)

R&D Systems | Catalog # MAB10077

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 976128
View all TDAG8/GPR65 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Met1-Glu337
Accession # Q8IYL9

Specificity

Detects human TDAG8/GPR65 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TDAG8/GPR65 Antibody (976128)

Detection of Human TDAG8/GPR65 antibody by Western Blot.

Detection of Human TDAG8/GPR65 by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TDAG8/GPR65. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TDAG8/GPR65 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TDAG8/GPR65 in Transfected HEK293 Human Cell Line.

TDAG8/GPR65 was detected in immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line (left panel, positive stain) and non-transfected HEK293 human embryonic kidney cell line (right panel, negative stain) using Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TDAG8/GPR65 Antibody (976128)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line

Western Blot

2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with human TDAG8/GPR65

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TDAG8/GPR65

Human G Protein-Coupled Receptor 65 (GPR65), also known as T-Cell Death-Associated Gene 8 Protein (TDAG8) and Psychosine Receptor, is a 337 aminoacids proton-sensing GPCR primarily expressed in lymphoid tissues and is uniquely expressed in Th17 cells; mice deficient in GPR65 are resistant to autoimmunity. GPR65 senses pH by protonation of histidine residues on its extracellular domain. Many pH-sensing G protein-coupled receptors have emerged as potential therapeutic agents in conditions that involve the response to acidotic stress.

Long Name

G Protein-coupled Receptor 65

Alternate Names

GPR65, Psychosine Receptor

Entrez Gene IDs

8477 (Human); 14744 (Mouse); 299242 (Rat)

Gene Symbol

GPR65

UniProt

Q8IYL9 (Human)

Additional TDAG8/GPR65 Products

Product Documents for Human TDAG8/GPR65 Antibody (976128)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TDAG8/GPR65 Antibody (976128)

For research use only

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Protocols

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