Human TGF-beta RII Antibody [Biotin]

R&D Systems | Catalog # BAF241

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Flow Cytometry, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TGF-beta RII Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RII
Ile24-Asp159
Accession # P37173

Specificity

Detects human TGF-beta  RII in ELISAs and Western blots. In sandwich immunoassays, approximately 25% cross-reactivity with recombinant human (rh) TGF-beta RIIb is observed and less than 0.05% cross-reactivity with rhTGF-beta RI, rhTGF-beta RIII, recombinant mouse TGF-beta RII, and rhTGF-beta 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TGF-beta RII Antibody [Biotin]

Human TGF-beta RII ELISA Standard Curve

Human TGF-beta RII ELISA Standard Curve

Recombinant Human TGF‑ beta  RII (Catalog # 241-R2) was serially diluted and captured by Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TGF-beta RII Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta  RII (Catalog # 241-R2)

Human TGF-beta RII Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TGF‑ beta  RII Antibody (Catalog # AF-241-NA)
  • Standard: Recombinant Human TGF-beta RII Protein (Catalog # 241-R2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.8 using BAF241 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References

  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

P37173

Additional TGF-beta RII Products

Product Documents for Human TGF-beta RII Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TGF-beta RII Antibody [Biotin]

For research use only

Citations for Human TGF-beta RII Antibody [Biotin]

Customer Reviews for Human TGF-beta RII Antibody [Biotin] (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human TGF-beta  RII Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/26/2021
    Human TGF-beta RII Antibody [Biotin] BAF241
  • Human TGF-beta  RII Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/17/2021
    Detect protein binding by ELISA. Used as secondary antibody
    Immobilized different doses of TGFb3, washed, and incubated with TBRII (20nM). Detect TBRII interaction, w/ Anti-TBRII-biotin followed by strepavidin-HRP.
    Human TGF-beta RII Antibody [Biotin] BAF241
  • Human TGF-beta  RII Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/16/2019
    Human TGF-beta RII Antibody [Biotin] BAF241
  • Human TGF-beta  RII Biotinylated Antibody
    Name: Jenna Nguyen
    Application: Western Blot
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 05/07/2019

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