Human Thrombopoietin/Tpo Antibody (34817)
R&D Systems | Catalog # MAB288
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 34817
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo
Ser22-Gly353
Accession # P40225
Ser22-Gly353
Accession # P40225
Specificity
Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Thrombopoietin/Tpo Antibody (34817)
Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody.
Recombinant Human Thrombo-poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND50 is typically 5-10 µg/mL.
Human Thrombopoietin / Tpo ELISA Standard Curve
Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Thrombopoietin/Tpo Antibody (34817)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.
Human Thrombopoietin/Tpo Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Thrombopoietin/Tpo
Alternate Names
MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo
Gene Symbol
THPO
UniProt
Additional Thrombopoietin/Tpo Products
Product Documents for Human Thrombopoietin/Tpo Antibody (34817)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Thrombopoietin/Tpo Antibody (34817)
For research use only
Related Research Areas
Citations for Human Thrombopoietin/Tpo Antibody (34817)
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