Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-1 (R&D Systems, Catalog # 970-TM)
Cys24-Ala207
Accession # Q6FGX5

Specificity

Detects human TIMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rmTIMP-1 and rhTIMP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TIMP-1 Antibody [Biotin]

Human TIMP-1 ELISA Standard Curve

Human TIMP-1 ELISA Standard Curve

Recombinant Human TIMP‑1 (Catalog # 970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TIMP-1 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TIMP‑1 (Catalog # 970-TM)

Human TIMP-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TIMP‑1 Antibody (Catalog # MAB970)
  • Standard: Recombinant Human TIMP-1 Protein, CF (Catalog # 970-TM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using BAF970 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol

TIMP1

UniProt

Q6FGX5

Additional TIMP-1 Products

Product Documents for Human TIMP-1 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TIMP-1 Antibody [Biotin]

For research use only

Citations for Human TIMP-1 Antibody [Biotin]

Customer Reviews for Human TIMP-1 Antibody [Biotin] (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human TIMP-1 Biotinylated Antibody
    Name: Hector Palacios
    Application: Immunoprecipitation
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/12/2018
  • Human TIMP-1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    This antibody was used as the detection molecule in a sandwich ELISA targeting TIMP-1. The antibody MAB970 was used as the capture molecule.
    Human TIMP-1 Antibody [Biotin] BAF970

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