Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation, Cell-based ELISA, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 89025
View all TIMP-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Cys27-Pro220
Accession # P16035

Specificity

Detects human TIMP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIMP-1 or recombinant mouse TIMP-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TIMP-2 Antibody (89025)

Detection of Human TIMP-2 antibody by Western Blot.

Detection of Human TIMP‑2 by Western Blot.

Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TIMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

TIMP‑2 in Human Ovarian Cancer Tissue.

TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of k7k9qrz3b3wvetd0cdxv.jpg by Western Blot

Detection of k7k9qrz3b3wvetd0cdxv.jpg by Western Blot

Expression of matrix remodeling proteins (MMP). (A) Immunoblots showing MMPs and TIMP‐2 expression (representative blots are shown). (B) Quantification of protein expression [A/B: n = 2 for MMP7 and MT1‐MMP, n = 3 for tissue inhibitor of MMPs (TIMP)1, n = 4 for MMP‐9 and n = 5 for MMP‐2; SEM, t‐test, P < 0.05, **P < 0.01]. (C) MMP activity in cell supernatants determined by gelatin‐zymography (n = 3, one representative zymogram is shown). (D) RNA expression of MMPs and TIMP‐2 in PAR, FR and FR/TK cells determined by RNASeq analysis (n = 3, ANOVA; n.s., not significant, *P < 0.05, ****P < 0.0001; FR, fast running shuttle cells; PAR, parental shuttle cells). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38115217), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TIMP-2 Antibody (89025)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

2 µg/mL
Sample: Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023), HeLa human cervical epithelial carcinoma cell line, and human placenta tissue

Reviewed Applications

Read 1 review rated 5 using MAB971 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Entrez Gene IDs

7077 (Human); 21858 (Mouse); 29543 (Rat)

Gene Symbol

TIMP2

UniProt

P16035

Additional TIMP-2 Products

Product Documents for Human TIMP-2 Antibody (89025)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TIMP-2 Antibody (89025)

For research use only

Citations for Human TIMP-2 Antibody (89025)

Customer Reviews for Human TIMP-2 Antibody (89025) (1)

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  • Human TIMP-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 08/14/2019
    Human TIMP-2 Antibody (89025) MAB971

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Protocols

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