Human TNF RI/TNFRSF1A Antibody (925224)

Name: Human TNF RI/TNFRSF1A Antibody (925224)
Brand: R&D Systems
SKU: MAB6251
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # MAB6251

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Citations (4)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 925224

View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A
Arg235-Arg455
Accession # P19438

Specificity

Detects human TNF RI/TNFRSF1A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human TNF RI/TNFRSF1A Antibody (925224)

Detection of Human TNF RI/TNFRSF1A by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TNF RI/TNFRSF1A Antibody (925224)

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.