Human Trappin-2/Elafin Antibody [Biotin]

R&D Systems | Catalog # BAF1747

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Trappin-2/Elafin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trappin-2/Elafin (R&D Systems, Catalog # 1747-PI)
Ala23-Gln117
Accession # P19957

Specificity

Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM‑2, and human Neutrophil Elastase is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Trappin-2/Elafin Antibody [Biotin]

Human Trappin-2 / Elafin ELISA Standard Curve

Human Trappin-2 / Elafin ELISA Standard Curve

Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI) was serially diluted and captured by Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Trappin-2/Elafin Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI)

Human Trappin-2/Elafin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Trappin‑2/Elafin Antibody (Catalog # AF1747)
  • Standard: Recombinant Human Trappin-2/Elafin Protein, CF (Catalog # 1747-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF1747 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Trappin-2/Elafin

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72-117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally.

References

  1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Alternate Names

Elafin, ESI, PI3, SKALP, Trappin2

Entrez Gene IDs

5266 (Human)

Gene Symbol

PI3

UniProt

P19957

Additional Trappin-2/Elafin Products

Product Documents for Human Trappin-2/Elafin Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Trappin-2/Elafin Antibody [Biotin]

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Trappin-2/Elafin Antibody [Biotin]

Customer Reviews for Human Trappin-2/Elafin Antibody [Biotin] (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Trappin-2/Elafin Antibody [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human Trappin‑2/Elafin Biotinylated Antibody
    Name: Anonymous
    Application: Luminex Development
    Sample Tested: Cell culture supernatant and cervical vaginal lavage
    Species: Human
    Verified Customer | Posted 05/06/2016

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies