Human Ubiquitin Antibody (1002A)

R&D Systems | Catalog # MAB8595

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Bacteria, Yeast

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1002A
View all Ubiquitin Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Ubiquitin
Accession # P0CG47

Specificity

Detects human Ubiquitin in direct ELISAs

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Ubiquitin Antibody (1002A)

Detection of Human Ubiquitin antibody by Western Blot.

Detection of Human Ubiquitin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Ubiquitin at various molecular weights (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Ubiquitin antibody by Western Blot.

Detection of Ubiquitin by Western Blot.

Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 5 ng/lane. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Di-Ubiquitin as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Ubiquitin Antibody (1002A)

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG132

Reviewed Applications

Read 2 reviews rated 5 using MAB8595 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

UniProt

P0CG47

Additional Ubiquitin Products

Product Documents for Human Ubiquitin Antibody (1002A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ubiquitin Antibody (1002A)

For research use only

Citations for Human Ubiquitin Antibody (1002A)

Customer Reviews for Human Ubiquitin Antibody (1002A) (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Human Ubiquitin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/19/2025
    Human Ubiquitin Antibody (1002A) MAB8595
  • Human Ubiquitin Antibody
    Name: Alice Meroni
    Application: Western Blot
    Sample Tested: hTERT RPE-1
    Species: Human
    Verified Customer | Posted 06/14/2019
    1:5000 dilution in 5% milk in PBS-T 0.05% O/N incubation developed with Odyssey imaging system
    Human Ubiquitin Antibody (1002A) MAB8595

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