Human VPS35 Antibody (1114723)

R&D Systems | Catalog # MAB11770

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1114723
View all VPS35 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q96QK1

Specificity

Detects a synthetic peptide specific for human VPS35 around amino acid 790 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VPS35 Antibody (1114723)

Detection of VSP35 in human breast tumor

VSP35 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human VSP35 Monoclonal Antibody (Catalog # MAB11770) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane in breast tumor. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human VPS35 Antibody (1114723)

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast tumor

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VPS35

VPS35 (vacuolar protein sorting-associated protein 35) is a critical component of the retromer complex, which plays an essential role in intracellular trafficking and protein recycling. With a molecular weight of approximately 90 kDa, VPS35 is responsible for mediating the retrieval of proteins and receptors from endosomes to their target destinations, including the trans-Golgi network and the plasma membrane. This process is crucial for maintaining cellular homeostasis, membrane integrity, and signaling pathways. VPS35 is ubiquitously expressed in various tissues and is fundamental for normal cellular function, particularly neuronal health. Dysregulation or mutation of VPS35 has been strongly linked to neurodegenerative diseases, such as Parkinson's disease, due to its role in recycling transmembrane proteins that regulate synaptic and neuronal stability. Furthermore, emerging evidence suggests VPS35 involvement in cellular mechanisms related to autophagy, mitochondrial function, and inflammation. VPS35 appears to play a role in maintaining lysosome and mitochondria integrity, processes that are often disrupted in cancer cells to support their metabolic and invasive properties. Emerging evidence suggests that VPS35 dysregulation could also contribute to chemo-resistance in malignancies, including breast cancer. Because of its role in endosomal trafficking, neuronal health, and cancer biology, VPS35 represents a promising biomarker and therapeutic target for neurodegenerative disorders and oncological diseases.

References

  1. Burd, C. et al. (2014) Cold Sprin Harb Perspect Biol. 6(2):a016774.
  2. Fan, X. et al. (2025) J. Inflamm. Res. 18:4665-4680.
  3. Li, X. et al. (2021) Cancer Cell Int. 21(1):265.

Long Name

VPS35 retromer complex component

Alternate Names

MEM3, PARK17

Entrez Gene IDs

55737 (Human); 65114 (Mouse); 25479 (Rat)

Gene Symbol

VPS35

UniProt

Q96QK1 (Human)

Additional VPS35 Products

Product Documents for Human VPS35 Antibody (1114723)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VPS35 Antibody (1114723)

For research use only

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Protocols

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