Human WIF-1 Antibody

Detection of WIF-1 by Western Blot

Human WIF1 binds human Shh with high affinity. In pull-down assays, we have incubated rhWIF1 with rhShh, then the solution was mixed with anti-Shh (A) or anti-WIF1 antibodies (B). The mixtures were pipetted on Pierce Spin Columns filled with Pierce Protein A/G Plus Agarose. After washing, the bound proteins were eluted with Pierce Classic IP kit Elution Buffer (pH 2.8). Samples were analyzed by SDS/PAGE, and the proteins were visualized by Western blotting (WB) with specific antibodies against rhWIF1 and rhShh. In control experiments, we have incubated rhWIF1 with anti-Shh antibody and rhShh with anti-WIF1 antibody. After the affinity binding step samples were visualized by Western blotting with specific antibodies against rhWIF1 and rhShh. Lanes: input, sample applied to the column; unbound fraction; washing fractions, wash1, wash2; bound fraction. The numbers indicate the molecular mass values of proteins of the Page Ruler Plus Protein Ladder (MM). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34944004), licensed under a CC-BY license. Not internally tested by R&D Systems.