Human Xg Antibody

R&D Systems | Catalog # AF3699

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Xg Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Xg
Gln22-Lys142
Accession # P55808

Specificity

Detects human Xg in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Xg Antibody

Detection of Xg antibody in Human Erythrocytes antibody by Flow Cytometry.

Detection of Xg in Human Erythrocytes by Flow Cytometry.

Human blood erythrocytes were stained with Goat Anti-Human Xg Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human Xg Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human blood erythrocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human Xg

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Xg

Xg, also known as PBDX (pseudoautosomal boundary divided on the X chromosome), is a type I transmembrane glycoprotein that belongs to the CD99 family. It is an erythrocyte membrane protein that is used as the basis for the Xg blood group system. Mature Xg is a 26 kDa glycoprotein that consists of a 121 aa extracellular domain, a transmembrane segment, and a 17 aa cytoplasmic tail. Xg isoforms with either a 15 aa insertion or a deletion of nearly the entire ECD have been reported. Within the extracellular domain, human Xg shares 100% aa sequence identity with mouse Xg.

Long Name

Xg Blood Group

Alternate Names

PBDX, XGPY

Entrez Gene IDs

7499 (Human)

Gene Symbol

XG

UniProt

P55808

Additional Xg Products

Product Documents for Human Xg Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Xg Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

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