Human ZNF281 Antibody (801706)

R&D Systems | Catalog # MAB6350

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 801706
View all ZNF281 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZNF281
Asn681-Arg895
Accession # Q9Y2X9

Specificity

Detects human ZNF281 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ZNF281 Antibody (801706)

Detection of Human ZNF281 antibody by Western Blot.

Detection of Human ZNF281 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF281 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ZNF281 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

ZNF281 in BG01V Human Embryonic Stem Cells.

ZNF281 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human ZNF281 antibody by Simple WesternTM.

Detection of Human ZNF281 by Simple WesternTM.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.5 mg/mL. A specific band was detected for ZNF281 at approximately 107 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human ZNF281 Antibody (801706)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Simple Western

50 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZNF281

ZNF281 (Zinc Finger protein 28), also known as ZBP99 and GZP1, is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4-37), a poly-Pro motif (aa 90-96) and four consecutive C2H2-type zinc finger domains (aa 261-367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681-895, human ZFN281 shares 99% aa identity with mouse ZFN281.

Long Name

Zinc Finger Protein 281

Alternate Names

GZP1, ZBP-99, ZNP-99

Entrez Gene IDs

23528 (Human); 226442 (Mouse); 305083 (Rat)

Gene Symbol

ZNF281

UniProt

Q9Y2X9 (Human)

Additional ZNF281 Products

Product Documents for Human ZNF281 Antibody (801706)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZNF281 Antibody (801706)

For research use only

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Protocols

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