IFITM3/Fragilis Antibody

Novus Biologicals | Catalog # NB600-1534

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IFITM3/Fragilis Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of mouse fragilis was used as the immunogen.

Localization

Cell Membrane

Marker

Primordial Germ Cell Marker

Specificity

Fragilis - Germ Line Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFITM3/Fragilis Antibody

Western Blot: IFITM3/Fragilis Antibody [NB600-1534]

Western Blot: IFITM3/Fragilis Antibody [NB600-1534]

Western Blot: IFITM3/Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of immunizing peptide using Fragilis antibody at 1 ug/ml.
Simple Western: IFITM3/Fragilis Antibody [NB600-1534]

Simple Western: IFITM3/Fragilis Antibody [NB600-1534]

Simple Western: IFITM3/Fragilis Antibody [NB600-1534] - Simple Western lane view shows a specific band for Fragilis in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for IFITM3/Fragilis Antibody

Application
Recommended Usage

Simple Western

1:10

Western Blot

0.5-2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFITM3/Fragilis

Principal Names: Interferon-induced transmembrane protein 3(Ifitm3); Fragilis Official Gene Symbol: Ifitm3 Gen Bank Accession Number: NM_025378 Gene ID: 66141(Mouse) Gene Map Locus: 7F5 (Mouse) Fragilis is an IFN-induced transmembrane protein that belongs to the highly conserved fragilis protein family. It consists of an N-terminal signal sequence, 2 putative transmembrane domains, 3 potential phosphorylation sites and C-terminal Leucine-zipper. It is expressed in early embryos and nascent primordial germ cells and is associated with Germ cell specification and development. It is cell surface protein and might be important in mediating interactions amongst germ cells and their surrounding neighbours. It is also suggested that it might be involved in anti-proliferative signaling and homotypic cell adhesion.

Long Name

Interferon-induced Transmembrane Protein 3

Alternate Names

Fragilis, IP15, mil-1

Gene Symbol

IFITM3

UniProt

Q9CQW9

Additional IFITM3/Fragilis Products

Product Documents for IFITM3/Fragilis Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IFITM3/Fragilis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Primordial and Mature Germ Cells

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