Q: Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?

IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81044

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Block/Neutralize, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # D9D10

Format

Azide and BSA Free

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

FPLC-purified natural human IFN-gamma. Refer to the original article [PMID: 3114127]

Reactivity Notes

Cross Reactivity: Baboon

Specificity

Binds human IFN-gamma, a dimerized soluble cytokine that is the only member of the type II class of interferons.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

Western Blot: IFN-gamma Antibody (D9D10)ChimericAzide and BSA Free [NBP2-81044]

Western Blot: IFN-gamma Antibody (D9D10) [NBP2-81044] - Western Blot using anti-IFN gamma antibody (D9D10) [NBP2-81044]. Human thymus lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of D9D10 [NBP2-81044] at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for IFN gamma is 19.3kDa, though the form shown here is likely glycosylated. NBP2-81044 successfully detected IFN gamma in human thymus samples.

Applications for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody can be used to detect both native and recombinant intereferon gamma (IFNg). It is reported only to bind to the functional, dimeric form of IFNg.

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IFN-gamma

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.