IgA Antibody (JM10-42)

Novus Biologicals | Catalog # NBP2-66901

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM10-42 expressed in HEK293
View all IgA Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length protein of Human IgA aa 1-353 / 353. (SwissProt: P01876 Human; SwissProt: P01877 Human)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgA Antibody (JM10-42)

Western Blot: IgA Antibody (JM10-42) [NBP2-66901]

Western Blot: IgA Antibody (JM10-42) [NBP2-66901]

Western Blot: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901]

Immunocytochemistry/ Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901]

Immunocytochemistry/Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901] - Staining IgA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. Counter stained with hematoxylin.
IgA Antibody (JM10-42) Western Blot: IgA Antibody (JM10-42) [NBP2-66901]

Western Blot: IgA Antibody (JM10-42) [NBP2-66901]

Western blot analysis of Human plasma with NBP2-66901 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 12s Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66901, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42.8 kDa Observed band size: 60 kDa

Applications for IgA Antibody (JM10-42)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgA

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Long Name

Immunoglobulin A

Alternate Names

Immunoglobulin A

Gene Symbol

IGHA1

Additional IgA Products

Product Documents for IgA Antibody (JM10-42)

Certificate of Analysis

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Product Specific Notices for IgA Antibody (JM10-42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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