Key Product Details

Species Reactivity

Porcine

Applications

Immunohistochemistry, Western Blot, ELISA, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Porcine IgG (H+L) Secondary Antibody was produced by repeated immunization with swine whole IgG molecule in goat.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Swine IgG and Swine Serum.

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Porcine IgG (H+L) Secondary Antibody

Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.
Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1,000 o/n at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.
Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none. Image from the Biotin version of this antibody.
Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the FITC version of this antibody.
Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Texas Red swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the Texas Red version of this antibody.
Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Dot Blot of Goat anti-Porcine IgG (H+L) Secondary Antibody [Alkaline Phosphatase]. Antigen: Swine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.
Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Load: 200 ug followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Fluorescien swine secondary antibody at 1:1,000 for 60 min at RT. Image from the FITC version of this antibody.
Goat anti-Porcine IgG (H+L) Secondary Antibody

Goat anti-Porcine IgG (H+L) Secondary Antibody

Western Blot of Goat anti-Swine IgG antibody. Lane 1: Swine IgG

Applications for Goat anti-Porcine IgG (H+L) Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product is tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Porcine IgG (H+L) Secondary Antibody

Certificate of Analysis

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Product Specific Notices for Goat anti-Porcine IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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