IgM Antibody (II/41) - BSA Free

Novus Biologicals | Catalog # NBP1-43303

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # II/41

Format

BSA Free
View all IgM Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was mouse IgM.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for IgM Antibody (II/41) - BSA Free

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy7 conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 micrograms of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.06 micrograms of Anti-Mouse IgM PE-Cy7 (right). Cells in the ly
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Staining of C57BL/6 splenocytes with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse IgM Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Biotin conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE.
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of FITC conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of Anti-Mouse IgM FITC (right). Total viable cells were used for ana
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE conjugate of NBP1-43303. The II/41 antibody has been tested by flow cytometric mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^
Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) - BSA Free [NBP1-43303]

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy5 conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 ug of Rat IgG2a Isotype Control PE-Cy5 (left) or 0.06 ug of Anti-Mouse IgM PE-Cy5 (right). Total viable cells were used for

Applications for IgM Antibody (II/41) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgM

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (II/41) - BSA Free

Certificate of Analysis

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Product Specific Notices for IgM Antibody (II/41) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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