Recombinant Porcine IL-12 p70/IL-12A Protein

Novus Biologicals | Catalog # NBP3-57563

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all IL-12 p70 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 197 aa of Porcine IL-12 p70/IL-12A.

Source: Yeast

Amino Acid Sequence: IWELEKNVYV VELDWYPNAP GEMVVLTCNT PEEDGITWTS DQSSEVLGTG KTLTIHVKEF GDAGQYTCRK GGAVLSQSLL LLHKKEDGIW STDILKDQKE PKNKSFLKCE AKNYSGRFTC WWLTAISTDL KFSVKSSRGS TDPRGVTCGT ATLSEDLGEY KKYRVECQEG SACPAAEESL PIEVVLEAVH KLKYENYTSS FFIRDIIKPD PPKNLQLNPL KNSRHVEISW EYPDTWSTPH SYFSLMFGVQ VQGKNKREKK DKLFTDQISA KVTCHKDANI RVQARDRYYS SSWSEWASVS CN (302) LINKER RSLPATTAGP GMFKCLNHSQ NLLKAVSNTL QKAKQTLEFY SCTSEEIDHE DITKDKTSTV EACLPLELAT NESCLAARET SLITNGNCLT SGKTSFMTTL CLSSIYEDLK MYHVEFQAMN AKLLMDPKRQ IFLDQNMLTA ITELMQALNF NSETVPQKPS LEELDFYKTK IKLCILLHAF RIRAVTIDRM MSYLNSS (197)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57563
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12 p70

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Recombinant Porcine IL-12 p70/IL-12A Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Porcine IL-12 p70/IL-12A Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Recombinant Porcine IL-12 p70/IL-12A Protein

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  • Q:

    Does the human IL-12 polyclonal antibody (Catalog # AB-219-NA) recognize IL-12 p70?

    A: In Western blots, this antibody recognizes the p70 heterodimer as well as the p40 and p35 subunits.

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