Recombinant Mouse IL-27 Protein

Novus Biologicals | Catalog # NBP3-57613

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all IL-27 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 206 aa of Mouse IL-27.

Source: Yeast

Amino Acid Sequence: FPTDPLSLQE LRREFTVSLY LARKLLSEVQ GYVHSFAESR LPGVNLDLLP LGYHLPNVSL TFQAWHHLSD SERLCFLATT LRPFPAMLGG LGTQGTWTSS EREQLWAMRL DLRDLHRHLR FQVLAAGFKC SKEEEDKEEE EEEEEEEKKL PLGALGGPNQ VSSQVSWPQL LYTYQLLHSL ELVLSRAVRD LLLLSLPRRP GSAWDS (206)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57613
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-27

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

Additional IL-27 Products

Product Documents for Recombinant Mouse IL-27 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-27 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Th1 Differentiation Pathway
Th1 Differentiation Pathway Thumbnail