IL-33 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24858

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine, Monkey

Predicted:

Primate (91%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-33 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 50-100 of human IL-33 was used as the immunogen for this antibody.

Reactivity Notes

Chimpanzee (91%), Bovine (85%), Mouse (78%).

Specificity

100% homologous in human (isoforms CRA_a and CRA_b).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-33 Antibody - BSA Free

Western Blot: IL-33 Antibody [NBP2-24858]

Western Blot: IL-33 Antibody [NBP2-24858]

Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.
Simple Western: IL-33 Antibody [NBP2-24858]

Simple Western: IL-33 Antibody [NBP2-24858]

Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for IL-33 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:200

Western Blot

0.5-2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 60 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% Gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-33

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

UniProt

O95760

Additional IL-33 Products

Product Documents for IL-33 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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