ILKAP/PP2C delta Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00036

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ILKAP/PP2C delta Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ILKAP/PP2C delta (Uniprot#: Q9H0C8; Met1-His392)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ILKAP/PP2C delta Antibody - Azide and BSA Free

Immunoprecipitation: ILKAP/PP2C delta Antibody [NBP3-00036]

Immunoprecipitation: ILKAP/PP2C delta Antibody [NBP3-00036]

Immunoprecipitation: ILKAP/PP2C delta Antibody [NBP3-00036] - ILKAP/PP2C delta was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 2 ul anti-ILKAP/PP2C delta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ILKAP/PP2C delta rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa
ILKAP/PP2C delta Antibody - Azide and BSA Free

Western Blot: ILKAP/PP2C delta Antibody [NBP3-00036] -

Western Blot: ILKAP/PP2C delta Antibody [NBP3-00036] - NBP3-00036 at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa

Applications for ILKAP/PP2C delta Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ILKAP/PP2C delta

ILKAP is encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Long Name

Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C

Alternate Names

PP2C delta

Gene Symbol

ILKAP

Additional ILKAP/PP2C delta Products

Product Documents for ILKAP/PP2C delta Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ILKAP/PP2C delta Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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