Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

Novus Biologicals | Catalog # NBP2-46018

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G9
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1 (wild type, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation, right lane "R132H") cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with NBP2-46018 (1:500) and then goat anti-mouse IgG-HRP (1:2000).
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-IDH1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100C for 10 min.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY IDH1 (RC210582, right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Analysis of extracts (35ug) from 9 different cell lines. HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100C for 10 min.
Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)[NBP2-46018]

Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)[NBP2-46018]Simple Western� analysis of endogenous protein TIA1 from HELA lysates (0.1 mg/mL) using TIA1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

Certificate of Analysis

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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