Kif2a Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83253

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Kif2a Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DNESVTVEWIENGDTKGKEIDLESIFSLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kif2a Antibody - BSA Free

Knockout Validated: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NBP1-83253) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253]

Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253]

Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253] - Staining of human testis shows nuclear positivity in cells of seminiferus ducts.
Western Blot: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253]

Western Blot: Kif2a Antibody [NBP1-83253] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Applications for Kif2a Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kif2a

Kif2a is a kinesin-like motor protein that depolymerizes microtubules. It is found in several vertebrate cell types but is most strongly expressed in neuronal cells. In dividing cells it is required for bipolar mitotic spindle formation and chromosome segregation. In neuronal cells it is involved in neurite outgrowth.

Alternate Names

HK2, KIF2kinesin-2, kinesin heavy chain member 2, kinesin heavy chain member 2A, Kinesin, heavy chain, 2, Kinesin-2, kinesin-like protein KIF2A, KNS2

Gene Symbol

KIF2A

Additional Kif2a Products

Product Documents for Kif2a Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Kif2a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Kif2a Antibody - BSA Free

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Protocols

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