KIF2C Antibody (1G2) - Azide and BSA Free

Novus Biologicals | Catalog # H00011004-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G2

Format

Azide and BSA Free
View all KIF2C Primary Antibodies »

Product Specifications

Immunogen

KIF2C (AAH14924, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTIQKQKRRSVNSKI

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasmic and Nuclear: Present throughout the cell cycle, associates with centromeres at early prophase, and remains associated with the centromere until after telophase.

Specificity

KIF2C - kinesin family member 2C

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for KIF2C Antibody (1G2) - Azide and BSA Free

Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01]

Immunocytochemistry/ Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01]

Immunocytochemistry/Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01]

Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01]

Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ug/ml.
Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi ( Cat # H00011004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01), clone 1G2 (Cat # H00011004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01]

Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody (M01), clone 1G2.Lane 1: KIF2C transfected lysate(81.3 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01]

Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01]

Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C transfected lysate using anti-KIF2C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KIF2C MaxPab rabbit polyclonal antibody.
Sandwich ELISA: KIF2C Antibody (1G2) [H00011004-M01] - Detection limit for recombinant GST tagged KIF2C is approximately 0.1ng/ml as a capture antibody.

Applications for KIF2C Antibody (1G2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KIF2C

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation.

Alternate Names

kinesin family member 2C, kinesin-like 6 (mitotic centromere-associated kinesin), Kinesin-like protein 6, kinesin-like protein KIF2C, MCAKKNSL6kinesin-like 6, Mitotic centromere-associated kinesin

Entrez Gene IDs

11004 (Human)

Gene Symbol

KIF2C

OMIM

604538 (Human)

UniProt

AAH14924 (Human)

Additional KIF2C Products

Product Documents for KIF2C Antibody (1G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KIF2C Antibody (1G2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KIF2C Antibody (1G2) - Azide and BSA Free

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Protocols

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FAQs

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