KLF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24749

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat

Predicted:

Bovine (97%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, In Situ Hybridization

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KLF4 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of human KLF4 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human and chimpanzee. Mouse reactivity reported in scientific publication.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF4 Antibody - BSA Free

Immunohistochemistry: KLF4 Antibody [NBP2-24749]

Immunohistochemistry: KLF4 Antibody [NBP2-24749]

KLF4-Antibody-Immunohistochemistry-NBP2-24749-img0006.jpg
Western Blot: KLF4 Antibody [NBP2-24749]

Western Blot: KLF4 Antibody [NBP2-24749]

Western Blot: KLF4 Antibody [NBP2-24749] - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-KLF4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry: KLF4 Antibody [NBP2-24749]

Immunohistochemistry: KLF4 Antibody [NBP2-24749]

KLF4-Antibody-Immunohistochemistry-NBP2-24749-img0007.jpg
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-24749-img0008.jpg
Flow Cytometry: KLF4 Antibody [NBP2-24749]

Flow Cytometry: KLF4 Antibody [NBP2-24749]

Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary.
Western Blot: KLF4 Antibody [NBP2-24749]

Western Blot: KLF4 Antibody [NBP2-24749]

Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). Goat anti-rabbit IgG HRP secondary and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. IHC-P image submitted by a verified customer review.
KLF4 Antibody

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative 3,3-diaminobenzidine immunohistochemical stained images of WHO grade I meningioma for OCT4 (A, brown), NANOG (B, purple), SOX2 (C, brown), KLF4 (D, purple) & c-MYC (E, brown) expressed on the endothelial (red arrows) & pericyte (black arrows) layers. Cell nuclei were counterstained with hematoxylin (A-E, blue). Original magnification: 400X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody

Western Blot: KLF4 Antibody [NBP2-24749] -

Western Blot: KLF4 Antibody [NBP2-24749] - Representative Western blot images of total protein extracted from four head & neck metastatic malignant melanoma-derived primary cell lines probed for induced pluripotent stem cell markers. Blots were probed for OCT4 (A), NANOG (B), SOX2 (C), KLF4 (D), & c-MYC (E) & detected with HRP conjugated goat anti-rabbit antibody. alpha -Tubulin was used as the loading control (F) & detected using HRP conjugated mouse IgGĸ binding protein. Arrows indicate the presence of the proteins with their expected band sizes. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32019273), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody

Immunohistochemistry: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry: KLF4 Antibody [NBP2-24749] - Representative immonoflourescence immunohistochemical stained images of WHO grade I meningioma demonstrating the expression of SOX2 (A, red), NANOG (B, red), KLF4 (C, red), OCT4 (D, green) & c-MYC (E, green) on both the SMA+ (A–C, red, red arrows) pericyte layer, & the ERG+ endothelial layer (D,E, red, white arrows). Cell nuclei were counterstained with 4′, 6′-diamino-2-phenylindole (A–F), blue. Original magnification: 600X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative colorimetric in situ hybridization stained images of WHO grade I meningioma demonstrating mRNA transcript expression for OCT4 (A, brown), NANOG (B, brown), SOX2 (C, brown), KLF4 (D, brown) & c-MYC (E, brown). Nuclei were counterstained with hematoxylin (A-E, blue). Orignal magnification: 1,000X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] -

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of SOX2 [(A,D), red], NANOG [(B,E), red], KLF4 [(C,F), red], & c-Myc [(D–F), green] on the cells within the tumor nests (TNs) [(A–F), thin arrows] & those within the peritumoral stroma [(A–F), arrowheads]. OCT4 [(A–C), green] was expressed on the SOX2+ [(A), green, arrowheads], NANOG+ [(B), green, arrowheads], & KLF4+ [(C), green, arrowheads] cells within the peritumoral stroma. There were also cells within the peritumoral stroma that expressed SOX2 [(A), red, thick arrows], NANOG [(B), red, thick arrows] & KLF4 [(C), red, thick arrows] that did not express OCT4. c-Myc was expressed by cells within the TNs [(D–F), green, arrows] & those within the peritumoral stroma [(D–F), arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed SOX2 [(D), red], NANOG [(E), red], & KLF4 [(F), red]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] -

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of SOX2 [(A,D), red], NANOG [(B,E), red], KLF4 [(C,F), red], & c-Myc [(D–F), green] on the cells within the tumor nests (TNs) [(A–F), thin arrows] & those within the peritumoral stroma [(A–F), arrowheads]. OCT4 [(A–C), green] was expressed on the SOX2+ [(A), green, arrowheads], NANOG+ [(B), green, arrowheads], & KLF4+ [(C), green, arrowheads] cells within the peritumoral stroma. There were also cells within the peritumoral stroma that expressed SOX2 [(A), red, thick arrows], NANOG [(B), red, thick arrows] & KLF4 [(C), red, thick arrows] that did not express OCT4. c-Myc was expressed by cells within the TNs [(D–F), green, arrows] & those within the peritumoral stroma [(D–F), arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed SOX2 [(D), red], NANOG [(E), red], & KLF4 [(F), red]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KLF4 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 34428252)

Immunohistochemistry

reported in scientific literature (PMID 34428252; 30901290)

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml

Reviewed Applications

Read 2 reviews rated 2.5 using NBP2-24749 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

UniProt

O43474 (Human)

Additional KLF4 Products

Product Documents for KLF4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KLF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLF4 Antibody - BSA Free

Customer Reviews for KLF4 Antibody - BSA Free (2)

2.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • KLF4 Antibody
    Name: Alena Zelinka
    Application: Immunohistochemistry-Frozen
    Sample Tested: skin
    Species: Bovine
    Verified Customer | Posted 12/03/2021
    Bovine skin stained with KLF4 antibody (1:50 dilution, original concentration = 1 mg/ml). 10 min pH 6 sodium citrate antigen retrieval used. No nuclear staining observed in epidermis.
    KLF4 Antibody - BSA Free NBP2-24749
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Liyong Zhang
    Application: Immunohistochemistry-Paraffin
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/21/2014
    KLF4 Antibody - BSA Free NBP2-24749

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