Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 20d5
View all KLRC1/2/3 Primary Antibodies »

Product Specifications

Immunogen

CHO cells transfected with B6 allele of NKG2A gene

Reactivity Notes

Mouse.

Specificity

Mouse NKG2 (Mr approx. 43 kDa)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for KLRC1/2/3 Antibody (20d5) [FITC]

Flow Cytometry: KLRC1/2/3 Antibody (20d5) [FITC] [NBP1-28101] - Analysis of C57BL/6 splenocytes by multiple staining.

Applications for KLRC1/2/3 Antibody (20d5) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KLRC1/2/3

The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b. (2, 3) NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). (1)

Alternate Names

CD159 antigen-like family member A, CD159a, CD159a antigen, C-lectin type II protein, killer cell lectin-like receptor subfamily C, member 1, MGC13374, natural killer cell lectin, natural killer group protein 2, NK cell receptor A, NKG2, NKG2-1/B activating NK receptor, NKG2-A, NKG2-A/B type II integral membrane protein, NKG2-A/B-activating NK receptor, NKG2-A/NKG2-B type II integral membrane protein, NKG2AMGC59791, NKG2-B

Gene Symbol

KLRC1

Additional KLRC1/2/3 Products

Product Documents for KLRC1/2/3 Antibody (20d5) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KLRC1/2/3 Antibody (20d5) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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