Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-44263

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Kynurenine 3-Monooxygenase/KMO Primary Antibodies »

Product Specifications

Immunogen

Kynurenine 3-Monooxygenase/Kynurenine 3-Monooxygenase/KMO antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 300-400aa of the mouse Kynurenine 3-Monooxygenase/KMO chain. (Uniprot: O15229)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Mouse based on 100% sequence homology. Cross-reactivity with Kynurenine 3-Monooxygenase/KMO from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44263]

Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44263]

Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44263] - Lane 1: Brain Extract. Load: 10 ug per lane. Primary antibody: KMO antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa for KMO. Other band(s): None.

Applications for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:20000-1:60000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Western Blot

1 ug/ml
Application Notes
This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Kynurenine 3-Monooxygenase/KMO

Kynurenine 3-monooxygenase (KMO) catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). The enzyme is required for synthesis of quinolinic acid. Quinolinic acid is a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development.

Alternate Names

KMO, Kynurenine 3Monooxygenase

Entrez Gene IDs

8564 (Human)

Gene Symbol

KMO

UniProt

O15229

Additional Kynurenine 3-Monooxygenase/KMO Products

Product Documents for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

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Protocols

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FAQs

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