LAT Antibody (24GB4940)

Novus Biologicals | Catalog # NBP3-51693

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4940
View all LAT Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human LAT (Uniprot: O43561)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAT Antibody (24GB4940)

LAT Antibody (24GB4940)

Flow Cytometry: LAT Antibody (24GB4940)[NBP3-51693] -

Validation of LAT knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LAT antibody (1:2,000) and analyzed using BD flow cytometer.
LAT Antibody (24GB4940)

Flow Cytometry: LAT Antibody (24GB4940)[NBP3-51693] -

Flow cytometric analysis of LATexpression in HT-1080 cells using LAT antibody (1:2,000). Green, isotype control; red, LAT.
LAT Antibody (24GB4940)

Western Blot: LAT Antibody (24GB4940)[NBP3-51693] -

Western blotting analysis using LAT antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
LAT Antibody (24GB4940)

Western Blot: LAT Antibody (24GB4940)[NBP3-51693] -

Western blotting analysis using LAT antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (linker for activation of T cells.

Applications for LAT Antibody (24GB4940)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LAT

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Gene Symbol

LAT

Additional LAT Products

Product Documents for LAT Antibody (24GB4940)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LAT Antibody (24GB4940)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LAT Antibody (24GB4940)

There are currently no reviews for this product. Be the first to review LAT Antibody (24GB4940) and earn rewards!

Have you used LAT Antibody (24GB4940)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies