Latrophilin 3/LPHN3 Antibody - BSA Free

Novus Biologicals | Catalog # NLS1138

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bat, Chicken, Equine, Monkey, Primate, Rabbit

Cited:

Human, Rat

Predicted:

Avian (100%), Bovine (95%), Opossum (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Western Blot, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Latrophilin 3/LPHN3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from C-terminus of human Latrophilin 3/LPHN3.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Gibbon (89%).

Localization

Plasma membrane

Specificity

Human LPHN3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Latrophilin 3/LPHN3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody - BSA Free [NLS1138]

Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody - BSA Free [NLS1138]

Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody [NLS1138] - Analysis of anti-LPHN3 / Latrophilin-3 antibody with human brain, glioblastoma.
Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody - BSA Free [NLS1138]

Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody - BSA Free [NLS1138]

Immunohistochemistry-Paraffin: Latrophilin 3/LPHN3 Antibody [NLS1138] - Analysis of anti-LPHN3 / Latrophilin-3 antibody with brain neurons at 2 ug/ml.
Latrophilin 3/LPHN3 Antibody - BSA Free

Western Blot: Latrophilin 3/LPHN3 Antibody - BSA Free [NLS1138] -

Confirmation of possible FLOT1-/FLOT2-positive early endosome cargo proteins.a Co-IP of FLOT1 with possible cargo proteins detected by DIA-LC-MS/MS analysis of FLOT1-/FLOT2-positive early endosomes (n = 1). b Average DIA protein abundance for LPHN1, LPHN2, LPHN3, CLTA, EEA1, and RAB5C. Data are presented as mean values + SD (n = 3, biologically independent samples over three independent experiments). Significance based on Student’s unpaired two-sided t test; range: ∗P < 0.05, ∗∗P < 0.01; exact P values: LPHN1 (0.022), LPHN2 (0.004), LPHN3 (0.044), CLTA (0.047), EEA1 (0.035), RAB5C (0.008). c Co-IP of FLOT1 and FLOT2 with LPHN1, LPHN2, and LPHN3. LDLR serves as marker for plasma membrane and clathrin-mediated endocytosis. d Immunostaining of HeLa cells for FLOT1 and FLOT2 in combination with LPHN1, LPHN2, and LPHN3 (n = 3). Lower panels show a zoom-in for regions indicated in full-size images. Mander’s coefficients were determined to assess signal overlap between individual populations. Data are presented as mean values + SD (n = 3, cells examined over one experiment). Profile plots indicate the degree of colocalization for individual vesicles. Scale bars = 5 uM; Cntrl. control, IN input, WA wash, EL eluate. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36266287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Latrophilin 3/LPHN3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Latrophilin 3/LPHN3

Latrophilin-3 is a brain-specific, Ca2+-independent Orphan-B Receptor with an unknown ligand. Unlike the other two latrophilins, Latrophilin-3 does not bind alpha-latrotoxin. Latrophilin-3, like its isoforms, shares homology with lectin, olfactomedin, and transmembrane domains, and possesses variable C-termini and various alternative-splicing sites. Latrophilin-3 has been reported in brain. ESTs have been isolated from B-cell/lung/testis, brain, ear, eye, heart, heart/melanocyte/uterus, kidney, lung, nerve, placenta, and testis libraries.

Alternate Names

CIRL3, LEC3, Lectomedin-3, LPHN3

Entrez Gene IDs

23284 (Human)

Gene Symbol

ADGRL3

UniProt

Q9HAR2 (Human)

Additional Latrophilin 3/LPHN3 Products

Product Documents for Latrophilin 3/LPHN3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Latrophilin 3/LPHN3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Latrophilin 3/LPHN3 Antibody - BSA Free

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Protocols

View specific protocols for Latrophilin 3/LPHN3 Antibody - BSA Free (NLS1138):

Immunohistochemistry Protocol for LPHN3 Antibody (NLS1138):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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