LATS1 Antibody

Novus Biologicals | Catalog # NB200-197

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LATS1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Large Tumor Suppressor homolog 1 using the numbering given in SwissProt entry O95835 (GeneID 9113).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LATS1 Antibody

Western Blot: LATS1 Antibody [NB200-197]

Western Blot: LATS1 Antibody [NB200-197]

Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: LATS1 Antibody [NB200-197]

Western Blot: LATS1 Antibody [NB200-197]

Western Blot: LATS1 Antibody [NB200-197] - Detection of Human LATS1 on HeLa whole cell lysate using NB200-197. LATS1 was also immunoprecipitated with another LATS1 antibody and NB200-198 using 3 mcg/mg lysate.

Applications for LATS1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 -5 ug/mg lysate
Application Notes
This antibody can be used in WesternBlot. Prepare working dilution immediately before use. Recommended Starting Dilutions*: WB: 1:5,000 - 1:15,000. *Optimal dilutions should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LATS1

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Alternate Names

EC 2.7.11, h-warts, Large tumor suppressor homolog 1, LATS (large tumor suppressor, Drosophila) homolog 1, LATS, large tumor suppressor, homolog 1 (Drosophila), serine/threonine-protein kinase LATS1, WARTS protein kinase, WARTSEC 2.7.11.1, wts

Entrez Gene IDs

9113 (Human)

Gene Symbol

LATS1

UniProt

O95835 (Human)

Additional LATS1 Products

Product Documents for LATS1 Antibody

Certificate of Analysis

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Product Specific Notices for LATS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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