LDB2 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-480

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LDB2 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified LDB2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2. (Uniprot: O55203)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for LDB2 Antibody - BSA Free

Western Blot: LDB2 Antibody [NB600-480]

Western Blot: LDB2 Antibody [NB600-480]

Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Immunohistochemistry: LDB2 Antibody [NB600-480]

Immunohistochemistry: LDB2 Antibody [NB600-480]

Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.

Applications for LDB2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: LDB2

LDB2 is a transcriptional activator that associates with the LIM homeoproteins and coordinates transcription. LIM homeoproteins and LDBs are involved in a variety of developmental processes. LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors which might implicate them in human phenotypes characterized by multiple affected sites.

Alternate Names

Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, CLIM1LDB1, LDB-2, LIM binding domain 2, LIM domain binding 2, LIM domain-binding factor CLIM1, LIM domain-binding factor-2, LIM domain-binding protein 2

Entrez Gene IDs

9079 (Human); 16826 (Mouse)

Gene Symbol

LDB2

UniProt

O55203 (Mouse)

Additional LDB2 Products

Product Documents for LDB2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LDB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LDB2 Antibody - BSA Free

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Protocols

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