Leptin R Antibody (JA73-01)

Novus Biologicals | Catalog # NBP2-66931

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA73-01 expressed in HEK293
View all Leptin R Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Leptin R aa 1041-1090 / 1165. (SwissProt: P48357 Human; SwissProt: P48356 Mouse; SwissProt: Q62959 Rat)

Localization

Secreted and Cell membrane. Basolateral cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leptin R Antibody (JA73-01)

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of Leptin Receptor on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunocytochemistry/ Immunofluorescence: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunocytochemistry/Immunofluorescence: Leptin R Antibody (JA73-01) [NBP2-66931] - Staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Flow Cytometry: Leptin R Antibody (JA73-01) [NBP2-66931]

Flow Cytometry: Leptin R Antibody (JA73-01) [NBP2-66931]

Flow Cytometry: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Western blot analysis of Leptin R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse liver tissue lysate
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931]

Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Leptin R Antibody (JA73-01) Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western blot analysis on different lysates with NBP2-66931 at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate(20 µg/Lane) Lane 2: Mouse brain tissue lysate(40 µg/Lane) Lane 3: Rat brain tissue lysate(40 µg/Lane) Exposure time: 3 minutine Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66931, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 132 kDa Observed band size: 132 kDa
Leptin R Antibody (JA73-01) Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931]

Western blot analysis on different lysates with NBP2-66931 at 1/1,000 dilution. Lane 1: K-562 cell lysate (16 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (32 µg/Lane) Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66931 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Applications for Leptin R Antibody (JA73-01)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:1,000

Immunohistochemistry-Paraffin

1:50-1:1,000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leptin R

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Gene Symbol

LEPR

Additional Leptin R Products

Product Documents for Leptin R Antibody (JA73-01)

Certificate of Analysis

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Product Specific Notices for Leptin R Antibody (JA73-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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