LIN-28B Antibody (24GB7210)

Novus Biologicals | Catalog # NBP3-51681

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 24GB7210
View all LIN-28B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human LIN-28B (Uniprot: Q6ZN17)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for LIN-28B Antibody (24GB7210)

LIN-28B Antibody (24GB7210)

Immunocytochemistry/ Immunofluorescence: LIN-28B Antibody (24GB7210)[NBP3-51681] -

Immunocytochemical staining of HAP-1 cells with LIN-28B antibody (1:1,000). Nuclei were stained blue with DAPI;LIN-28B was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
LIN-28B Antibody (24GB7210)

Flow Cytometry: LIN-28B Antibody (24GB7210)[NBP3-51681] -

Flow cytometric analysis of LIN-28Bexpression in HAP-1 cells using LIN-28B antibody (1:2,000). Green, isotype control; red, lin-28 homolog B.
LIN-28B Antibody (24GB7210)

Flow Cytometry: LIN-28B Antibody (24GB7210)[NBP3-51681] -

Validation of LIN-28B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LIN-28B antibody (1:2,000) and analyzed using BD flow cytometer.
LIN-28B Antibody (24GB7210)

Western Blot: LIN-28B Antibody (24GB7210)[NBP3-51681] -

Western blotting analysis using LIN-28B antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for LIN-28B Antibody (24GB7210)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIN-28B

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Gene Symbol

LIN28B

Additional LIN-28B Products

Product Documents for LIN-28B Antibody (24GB7210)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LIN-28B Antibody (24GB7210)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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