Ly-6C Antibody (PSH04-51)

Novus Biologicals | Catalog # NBP3-32548

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH04-51 expressed in HEK293
View all Ly-6C Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within mouse Ly-6C aa 51-100 / 131. (Uniprot: P0CW02)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ly-6C Antibody (PSH04-51)

Ly-6C Antibody (PSH04-51) Western Blot: Ly-6C Antibody (PSH04-51) [NBP3-32548]

Western Blot: Ly-6C Antibody (PSH04-51) [NBP3-32548]

Western blot analysis on different lysates with NBP3-32548 at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse testis tissue lysate (low expression) Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32548 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Ly-6C Antibody (PSH04-51)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6C

Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes. (1-4)

Alternate Names

Ly 6c, Ly6c protein, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen Ly 6C

Gene Symbol

Ly6c1

Additional Ly-6C Products

Product Documents for Ly-6C Antibody (PSH04-51)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ly-6C Antibody (PSH04-51)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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