Lyn Antibody

Novus Biologicals | Catalog # NBP2-17189

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Mouse (95%), Rat (95%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Lyn Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%), Chicken (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lyn Antibody

Immunocytochemistry/ Immunofluorescence: Lyn Antibody [NBP2-17189]

Immunocytochemistry/ Immunofluorescence: Lyn Antibody [NBP2-17189]

Immunocytochemistry/Immunofluorescence: Lyn Antibody [NBP2-17189] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Lyn Antibody

Western Blot: Lyn Antibody [NBP2-17189] -

Western Blot: Lyn Antibody [NBP2-17189] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lyn Antibody

Western Blot: Lyn Antibody [NBP2-17189] -

LYN antibody detects LYN protein by Western blot analysis.
A. 30 ug THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (NBP2-17189) dilution: 1:1000
Lyn Antibody

Immunoprecipitation: Lyn Antibody [NBP2-17189] -

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. 40 ug K562 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of LYN protein by 4 ug LYN antibody (NBP2-17189)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (NBP2-17189) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for Lyn Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lyn

Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino-terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. The human Lyn gene maps to chromosome 8q13 and encodes a 505 amino acid protein. Hematopoietic tissues predominantly express Lyn, which influences normal immunoglobulin production and regulation.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Alternate Names

Hck-2, JTK8, v-yes-1

Gene Symbol

LYN

UniProt

P07948

Additional Lyn Products

Product Documents for Lyn Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lyn Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

B Cell Activation
Intracellular Kinases

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Protocols

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FAQs

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