Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Novus Biologicals | Catalog # NB110-40585

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Lysine (K)-specific Demethylase 4A/KDM4A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1010 and the C-terminus (residue 1064) of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NB110-40585-img0006.jpg
Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585]

Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40585 used for IP at 6 ug per reaction. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584. For blotting immunoprecipitated Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A, NB110-40585 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] -

Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. (A) Left; HDF were subjected to IR (10 Gy) & allowed to recover for 1 h. Association between A-type lamins & 53BP1 was assessed by immunoprecipitation of endogenous lamin A/C followed by immunoblotting with 53BP1 & lamin A/C antibodies. WCE, whole cell extract, IP: immunoprecipitates. WCE represents 5% input. Right; endogenous lamin A/C was immunoprecipitated & supernatants or pellets were analysed by immunoblotting for interaction with JMJD2A. (B) U2OS/GFP-lamin A cells were pretreated with caffeine (20 mm) for 1 h before exposure to IR (10 Gy, 1 h recovery). Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, & bound complexes were then analysed by immunoblotting using 53BP1 & GFP antibodies. WCE represents 1% input. (C) As in (C) except cells were pretreated with 10 μm ATMi for 1 h before IR. WCE represents 1% input. (D) U2OS/GFP-lamin A cells were subjected to laser micro-irradiation, fixed 1 h later & immunostained with gamma -H2AX antibody. Scale bar, 10 μm. (E) U2OS cells were transfected with siCTRL or siLMNA & subjected to laser micro-irradiation, fixed 15 min later & then processed for immunofluorescence with gamma -H2AX & 53BP1 antibodies. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25645366), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] -

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] -

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] -

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 22373579), Use Use in ICC/IF reported in scientific literature ( 25645366).

Reviewed Applications

Read 2 reviews rated 5 using NB110-40585 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lysine (K)-specific Demethylase 4A/KDM4A

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Entrez Gene IDs

9682 (Human); 230674 (Mouse)

Gene Symbol

KDM4A

UniProt

NP_055478.1 (Human)

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Customer Reviews for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (2)

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  • Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody
    Name: Anonymous
    Application: Immunofluorescent staining
    Sample Tested: epithelial cells
    Species: Human
    Verified Customer | Posted 07/10/2018
    Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody NB110-40585
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cell lysates
    Species: Human
    Verified Customer | Posted 01/11/2012
    Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody NB110-40585

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