Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97)

Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495]

Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B was immunoprecipitated in 0.2mg A549 cell lysate with NBP3-32495 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32495 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: A549 cell lysate (input)
Lane 2: IP in A549 cell lysate
Lane 3: Rabbit IgG in A549 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495]

Western blot analysis on different lysates with NBP3-32495 at 1/2,000 dilution. Lane 1: BT-20 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: AGS cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 176 kDa Observed band size: 145 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32495 at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.