M-CSF Antibody Pair [Biotin]

Novus Biologicals | Catalog # NBP3-57744

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Sample Type

Cell culture supernatants from dogs (Canis lupus familiaris). Additional biological sample types reported in the literature: serum, plasma, PBMC culture supernatants, bone marrow-derived cell cultures, and tissue lysates. (100 ul)

Reactivity

Canine
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Product Specifications

Description

Contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of components are not matched, and individual components may be purchased separately. This product is for research use only.

Sample Volume Required

100 ul

Conjugate

Biotin

Scientific Data Images for M-CSF Antibody Pair [Biotin]

M-CSF Antibody Pair [Biotin]

ELISA: M-CSF Antibody Pair [Biotin] [NBP3-57744] -

Representative standard curve for M-CSF Antibody Pair [Biotin] (NBP3-57744)

Kit Contents for M-CSF Antibody Pair [Biotin]

  • Capture Antibody (1 Set = <= 10 Plates; 2 Set = <= 20 Plates)
  • Detection Antibody (Biotinylated)
  • Standard: (Catalog # <a href= "/products/m-csf-recombinant-protein_nbp3-57509" >NBP3-57509</a>)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: M-CSF

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for M-CSF Antibody Pair [Biotin]

Certificate of Analysis

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Product Specific Notices for M-CSF Antibody Pair [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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