MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00007867-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Sandwich ELISA, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F4

Format

Azide and BSA Free
View all MAPKAP Kinase 3 Primary Antibodies »

Product Specifications

Immunogen

MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MAPKAPK3 - mitogen-activated protein kinase-activated protein kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4. Analysis of MAPKAPK3 expression in NIH/3T3.
Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4 Analysis of MAPKAPK3 expression in HeLa.
Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Immunoprecipitation: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01]

Immunoprecipitation: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK3 monoclonal antibody.
Sandwich ELISA: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.

Applications for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:50
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: separated by Size, Charge, antibody dilution of 1:50, apparent MW was 50 kDa

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAPKAP Kinase 3

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Alternate Names

3PKEC 2.7.11.1, Chromosome 3p kinase, EC 2.7.11, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MAPKAP33pK, MAPKAPK-3, mitogen-activated protein kinase-activated protein kinase 3

Entrez Gene IDs

7867 (Human)

Gene Symbol

MAPKAPK3

OMIM

602130 (Human)

UniProt

AAH01662 (Human)

Additional MAPKAP Kinase 3 Products

Product Documents for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

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FAQs

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