Mcl-1 Antibody (CL1128) - BSA Free

Novus Biologicals | Catalog # NBP2-52968

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1128

Format

BSA Free
View all Mcl-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q07820, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mcl-1 Antibody (CL1128) - BSA Free

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968]

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968]

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of lymph node in human colon shows strong positivity in the reaction centrum.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human breast cancer shows strong positivity in tumor cells.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human cerebellum shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human tonsil shows strong immunoreactivity in the reaction centrum.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of stomach cancer shows cytoplasmic immunoreactivity in tumor cells.

Applications for Mcl-1 Antibody (CL1128) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reviewed Applications

Read 1 review rated 5 using NBP2-52968 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (CL1128) - BSA Free

Certificate of Analysis

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Product Specific Notices for Mcl-1 Antibody (CL1128) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Mcl-1 Antibody (CL1128) - BSA Free (1)

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  • Mcl-1 Antibody (CL1128)
    Name: Kristen Gumz
    Application: ELISA
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 04/28/2017

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Protocols

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