MED13 Antibody
Novus Biologicals | Catalog # NB100-60642
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Primate
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human thyroid hormone receptor associated protein 1 (thyroid hormone receptor-associated protein, 240 kDa) using the numbering given in entry NP_005112.2 (GeneID 996
Reactivity Notes
Primate reactivity reported in scientific literature (PMID: 24147125).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MED13 Antibody
Western Blot: MED13 Antibody [NB100-60642] -
TAP-n208 associates with both TFIID and Mediator.(A) Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS), TAP-wtICP4 (TAP), the C-terminal truncation mutant (n208) or a TAP-tagged C-terminal truncation mutant (TAP-n208) expressing viruses. Antibodies directed against ICP4, TAF1 and TBP of TFIID, and Med1 of Mediator were used. (B) Quantification of the western blots in (A). Images were quantified using the LiCOR Odyssey infrared imager and normalized to the amount of ICP4 present within the sample. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24147125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: MED13 Antibody [NB100-60642] -
TFIID and Mediator display different temporal associations with ICP4.(A) Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at either 3 hpi or 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against ICP4, TAF1 and TBP of TFIID, and Med1 of Mediator were used. (B) Quantification of the western blots in (A). Images were quantified using the LiCOR Odyssey infrared imager and normalized to the amount of ICP4 present within the sample. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24147125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for MED13 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MED13
Alternate Names
Activator-recruited cofactor 250 kDa component, KIAA0593HSPC221, mediator complex subunit 13ARC250, mediator of RNA polymerase II transcription, subunit 13 homolog, THRAP1mediator of RNA polymerase II transcription subunit 13, thyroid hormone receptor associated protein 1, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, thyroid hormone receptor-associated protein complex component TRAP240, thyroid hormone receptor-associated protein, 240 kDa subunit, Trap240, TRAP240DRIP250, Vitamin D3 receptor-interacting protein complex component DRIP250
Entrez Gene IDs
9969 (Human)
Gene Symbol
MED13
UniProt
Additional MED13 Products
Product Documents for MED13 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MED13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MED13 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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