MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-20686
![MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-2711202419553094.jpg "Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
Loading...
Product Specifications
Immunogen
MEK1/2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/2.
Modification
p Ser221, p Ser217
Specificity
This antibody detects endogenous level of MEK1/2 only when phosphorylated at serine 217/221.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -
Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).![MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-271120242074623.jpg "Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -")
Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -
Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/2 (p Ser221, p Ser217)Applications for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:100
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Epitope affinity purified
Formulation
PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: MEK1/MEK2
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbol
MAP2K1
Additional MEK1/MEK2 Products
Product Documents for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
There are currently no reviews for this product. Be the first to review MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free and earn rewards!
Have you used MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
