METTL3 Antibody

Name: METTL3 Antibody
Brand: Novus Biologicals
SKU: NB100-77310
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-77310

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all METTL3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for METTL3 Antibody

Western Blot: Methyltransferase like 3 Antibody [NB100-77310]

Western Blot: Methyltransferase like 3 Antibody [NB100-77310] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL3/MT-A70 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77310]

Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77310] - Detection of human METTL3/MT-A70 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody NB100-77310 used for IP at 3 ug per reaction. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody NB100-77311. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for METTL3 Antibody

Application

Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: METTL3

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Long Name

Methyltransferase like 3

Alternate Names

adoMet-binding subunit of the human mRNA (N6-adeno, hMETTL3, IME4, mRNA m(6)A methyltransferase, MT-A70, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, Spo8

Entrez Gene IDs

56339 (Human); 56335 (Mouse)

Gene Symbol

Q86U44

UniProt

Q86U44 (Human)

Additional METTL3 Products

Product Documents for METTL3 Antibody

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Product Specific Notices for METTL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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